Figure 3.

Quantitative expression of various reporter gene constructs carrying SM50 and Endo16 regulatory modules at the late gastrula stage. The number of CAT enzyme molecules per embryo was determined in CAT assays by comparison of the samples to standards of known CAT protein concentration. All data are averages, and vertical lines through the bars represent standard deviations (n = 4). The values measured separately for GBA–Bp·CAT and SM50·CAT (Left) or GDCBA–Bp·CAT and SM50·CAT (Right) are shown as white and black bars, respectively. These values were added, and the totals set to 100%. Experimental values obtained with GBA–SM50·CAT and GDCBA–SM50·CAT were compared with these totals and also expressed as percentages (gray bars).