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. 2007 Aug 14;4(8):e253. doi: 10.1371/journal.pmed.0040253

Figure 3. Polyclonal T Cells Infiltrate LCH Granuloma in Close Contact with LCs.

Figure 3

(A) Double immunohistochemical labeling of frozen sections from an eosinophilic granuloma with anti-CD3 (brown) and anti-CD1a antibodies (blue).

(B) T cell receptor gamma rearrangement was determined on the DNA extracted from frozen biopsies from four patients. Fluorescent profiles for Vgfl/Vg10 PCR using fluorescent Jg primers (JgP, red; Jg1/2, green; JgP1/2, blue) are shown; all the biopsies display a polyclonal profile in comparison to polyclonal and monoclonal positive controls.