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. 2007 Jul 10;5(8):e192. doi: 10.1371/journal.pbio.0050192

Figure 4. Colocalization of IE Gene Transcription Signals with Igh .

Figure 4

(A and B) Double-label RNA FISH in stimulated splenic B cells for Igh (red) and Fos (green) (A) or Igh (red) and Myc (green) (B). Scale bar, 2 μm.

(C) The percentages of Igh signals with visibly overlapping (colocalizing) Fos or Myc signals, before and after B cell stimulation for the times indicated in minutes. One hundred nuclei were assessed for signals in each case. There are significantly more colocalizing signals in stimulated cells compared to unstimulated for Fos (0 min versus 5 min, p = 1.2 × 10−4; versus 10 min, p = 3.3 × 10−4; and versus 15 min, 1.4 × 10−3) and Myc (0 min versus 5 min, p = 2.5 × 10−3; versus 10 min, 4.2 × 10−3; and versus 15 min, p = 1.9 × 10−4).

(D) A single optical section of a triple-label, RNA immuno-FISH on stimulated B cells showing Igh (red) and Myc (green) transcription, and RNAPII foci (blue). Images on right are enlargements of colocalized transcription signals associating with the same RNAPII focus. Scale bar, 2 μm.