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. Author manuscript; available in PMC: 2008 Jul 20.
Published in final edited form as: Biochem Biophys Res Commun. 2007 May 14;359(1):63–69. doi: 10.1016/j.bbrc.2007.05.041

Figure 2.

Figure 2

Effect of the peptides on the migration of HUVECs in a modified Boyden chamber migration assay. Endothelial cells were allowed to migrate for 20 h in the presence of 20 ng/ml VEGF and 30 μg/ml peptide solution, then stained with calcein and counted. The fluorescent signal was initially scaled so that 0% represents the negative control (endothelial cells in serum- and growth factor-free medium) and 100% the positive control (migration in the presence of 20 ng/ml VEGF). Tthe percentage migration inhibition, shown in the figure, was calculated. Vertical bars indicate the standard error. All values are significantly different from 0% at p<0.001, except those marked by NS (non-significant). In all cases, the standard error for the controls was <5% (n=8).