Table 1.
mRNA expression of genes involved in the PLA2/COX pathway in the rat cerebral cortex during aging using quantitative real–time PCR.
Genes1 | 4 months (n=6) | 12 months (n=6) | 24 months (n=6) | 30 months (n=5) |
---|---|---|---|---|
COX-1 | 100 ± 2 | 100 ± 5 | 105 ± 9 | 108 ±7 |
COX-2 | 100 ± 5 | 126 ±12 | 100 ± 3 | 97 ± 8 |
cPLA2 | 101 ± 5 | 113 ± 6 | 118 ± 6 | 105 ± 7 |
iPLA2 | 100 ± 3 | 96 ± 4 | 90 ± 6 | 88 ± 6 |
GFAP | 101 ± 6 | 122 ± 4 | 206 ± 12 *** | 241 ± 11 *** |
Data (mean ± SEM, n=5–6) are normalized to the level of the internal control, GAPDH, and are expressed as the percentage of values in the 4 month-old rats. p values are expressed as the comparison of aged rats with 4-month-old rats using One-Way ANOVA
p<0.01; post-hoc Bonferroni test.