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. Author manuscript; available in PMC: 2008 Jul 18.
Published in final edited form as: Cancer Lett. 2007 Feb 14;252(2):299–306. doi: 10.1016/j.canlet.2007.01.001

Fig.2.

Fig.2

Inhibition of AR prevents TNF-α -induced Cox activity in Caco-2 cells. (A) Cox activity was determined by using Enzyme Immuno Assay kit. (B-C) Pooled cell extracts from 3 independent experimental sets were subjected to SDS-PAGE and Western blots were developed using antibodies against Cox-2 (B), Cox-1 (C) and GAPDH (D). (E) Cox-2 mRNA expression was determined by Qiagen RT-PCR (F) mRNA loading control, β-actin. One unit of Cox activity is defined as the amount of enzyme that will cause the oxidation of 1 nmol of TMPD per minute at 25 °C. Bars represent mean ± S.E. (n = 4); #, p < 0.001 compared to control cells, *, p < 0.01 compared to cells treated with TNF-α alone.