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. Author manuscript; available in PMC: 2008 Jul 18.
Published in final edited form as: Cancer Lett. 2007 Feb 14;252(2):299–306. doi: 10.1016/j.canlet.2007.01.001

Fig.4.

Fig.4

Inhibition of AR abrogates TNF-α- induced PKC activation. Quiescent Caco-2 cells were preincubated with 20 μM sorbinil for 24 h followed by stimulation with TNF-α (2 nM) for 2 h. (A) PKC activity was determined by using Promega's total PKC assay system. (B & C) The pooled cell extracts from 3 independent experimental sets were subjected to SDS-PAGE and Western blots were developed using antibodies, phospho-PKC-β2 (B) and nonphospho-PKC-β2 (C). Bars represent mean ± S.E. (n = 4); #, p < 0.001 as compared to control cells. *, p < 0.001 compared to cells treated with TNF-α alone.