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. Author manuscript; available in PMC: 2008 Jul 18.
Published in final edited form as: Cancer Lett. 2007 Feb 14;252(2):299–306. doi: 10.1016/j.canlet.2007.01.001

Fig.5.

Fig.5

Inhibition of AR prevents lipid aldehyde-induced NF-κB activation and growth in Caco-2 cells. (A) Growth-arrested Caco-2 cells with or without sorbinil (20 μM) were incubated with 0.1 μM each of GS-HNE-ester and GS-DHN-ester for 24 h. The cell viability was determined by MTT assay. (B) The transiently transfected Caco-2 cells with pNF-κB secretary alkaline phosphatase reporter construct or control plasmid, pTALSEAP were incubated with 1 μM each of HNE, GS-HNE-ester or GS-DHN ester and alkaline phosphatase (SEAP) activity was measured as described in methods. Bars represent mean ± S.E. (n = 4); #, p < 0.001 as compared to control cells. *, p < 0.01 compared to cells treated with aldehydes alone.