Table 2.
The phylogenetic affiliations of the bacterial populations detected by PCR cloning from a Hudson River sediment microcosm (day 225) amended with 2,3,4,5-CB and 100 mg l−1 of bicarbonate.
| Clone | Sequence length | Clone frequency (%) | Phylogenetic relationship
|
||
|---|---|---|---|---|---|
| Species (accession No.) | % Identity | Phylum | |||
| Z9 | 852 | 3.7 | Clostridium cylindrosporum (Y18179) | 91.6 | Firmicutes |
| Z17 | 1476 | 7.4 | Environmental clone KD3-5 (AY188305) | 97.2 | Bacteroidetes |
| Z20 | 1415 | 3.7 | Chimeric | ||
| Z29 | 1447 | 11.1 | Dehalobacter restrictus (Y10164) | 99.5 | Firmicutes |
| Z30 | 815 | 3.7 | Environmental clone GR25 (AY150887) | 97.2 | Ambiguous |
| Z35 | 1456 | 7.4 | Sedimentibacter hydroxybenzoicus (L11305) | 93.7 | Firmicutes |
| Z39 | 873 | 3.7 | Pelospora glutarica (AJ251214) | 93.6 | Firmicutes |
| Z40 | 1446 | 3.7 | Dehalobacter restrictus (Y10164) | 99.0 | Firmicutes |
| Z42 | 1449 | 14.8 | Dehalococcoides strain CBDB1 (AF230641) | 99.8 | Chloroflexi |
| Z43 | 824 | 3.7 | Environmental clone DEL55 (AJ616285) | 97.9 | Planctomycetales |
| Z44 | 838 | 3.7 | Environmental clone NMW3.210WL (AY043958) | 87.5 | Ambiguous |
| Z45 | 853 | 3.7 | Environmental clone BIOEST-22 (AJ548911) | 98.3 | Firmicutes |
| Z52 | 849 | 3.7 | Sedimentibacter hydroxybenzoicus (L11305) | 92.1 | Firmicutes |
| Z54 | 862 | 11.1 | Clostridium sp. 9B4 (AY554416) | 96.8 | Firmicutes |
| Z57 | 820 | 3.7 | Environmental clone WCHB1-40 (AF050549) | 96.2 | Spirochaetes |
| Z60 | 853 | 3.7 | Arthrobacter globiformis (AB098573) | 99.5 | Actinobacteria |
| Z62 | 1470 | 3.7 | Environmental clone BSA1B-04 (AB175358) | 98.5 | Firmicutes |
| Z63 | 818 | 3.7 | Environmental clone BIOEST-22 (AJ548911) | 97.0 | Firmicutes |