Fig. 3.
Circular dichroism spectra for wild-type and mutant TyrH. A: Wild-type (———), T245P (- - -), and T463M (· · ··) TyrH. B: Wild-type (———), T283M (- - -), and R306H (· · ··) TyrH. Conditions: 5 μM enzyme, 50 mM MOPS, pH 7.0, 4°C. For the spectra in (B), the buffer also contained 10% glycerol.