. Author manuscript; available in PMC: 2007 Aug 13.

Published in final edited form as: Proteins. 2005 Jan 1;58(1):14–21. doi: 10.1002/prot.20293

TABLE I.

Kinetic Parameters of Wild-Type and Mutant Tyrosine Hydroxylase

Enzyme	K_tyr (μM)^a	K_ityr (μM)^a	K_BH4 (μM)^b	k_cat (min⁻¹)^a
Wild-type	46 ± 1	46 ± 1	13 ± 2	200 ± 2
T245P	66 ± 5	73 ± 7	14 ± 1	321 ± 8
T283M	39 ± 2	37 ± 2	10 ± 1	48 ± 1
R306H	42 ± 3	48 ± 3	10 ± 1	221 ± 5
T463M	43 ± 3	44 ± 3	9 ± 1	232 ± 6

Conditions: 300 μM BH₄, 0 to 150 μM tyrosine, 100 μg/mL catalase, 10 μM ferrous ammonium sulfate, 1 mM dithiothreitol, 50 mM HEPES, pH 7.0, 30°C.

Conditions: 30 μM tyrosine, 0 to 80 μM BH₄, 100 μg/mL catalase, 10 μM ferrous ammonium sulfate, 1 mM dithiothreitol, 50 mM HEPES, pH 7.0, 30°C.