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. Author manuscript; available in PMC: 2007 Aug 13.
Published in final edited form as: Anal Biochem. 2006 Nov 16;361(1):31–46. doi: 10.1016/j.ab.2006.10.042

Fig. 4.

Fig. 4

Transcription reactions with template DNAs bearing one (A), two (B) or multiple (C) T7 promoters were surface-mounted (on trimethyl surfaces) and stained with YOYO-1. A) Representative image from a reaction supported by the cosmid LANL-16c_380H5, a 44.675 kb template with one promoter 16 kb from one end. B) Representative image from a reaction supported by 1S1C_Tφ, which is 27.212 kb and has two promoters at 3.675 kb and 9.665 kb from one end. C) Representative images from a reaction supported by T7 genomic DNA, which is 39.937 kb and has 17 promoters. All template DNAs were linearized prior to transcription.