Table 1.
Thermodynamic parameters describing unfolding of wt A4 and dimer dissociation coupled with unfolding for C321S and F283L/C321S variants1
| Cm1 (M) | m1 | ΔG1 | Kd (nM)3 | Cm2 (M) | m2 | ΔG2 | |
|---|---|---|---|---|---|---|---|
| wt A4 | 3.20 ± 0.06 | 0.80 ± 0.1 | 2.6 ± 0.3 | N.A. | 4.84 ± 0.01 | 2.57 ± 0.03 | 12.4 ± 0.2 |
| C321S A4 | (2.75)2 | 1.20 ± 0.06 | 9.5 ± 0.45 | 90 ± 50 | (4.37)4 | 0.60 ± 0.03 | 2.61 ± 0.17 |
| F283L/C321S A4 | (1.35)2 | 2.50 ± 0.006 | 8.71 ± 0.02 | 350 ± 20 | (4.30)4 | 0.82 ± 0.01 | 3.63 ± 0.04 |
1
The units for m and ΔG are kcal l mol−2 and kcal mol−1, respectively (1 kcal = 4.187 kJ).
2
Apparent midpoint of second-order transition (N2 ⇔ 2M) from Figures 4B and 9B.
3
Dimer dissociation constant, Kd = exp(−ΔG1/RT), where R=1.987 10−3 kcal mol−1 K−1 is the gas constant.
4
Midoint of the unimolecular transition (M⇔U), Cm2 = ΔG2/m2.