Figure 3.

Paternal-specific DNA methylation of the M. spretus transgene. (A) The positions of BamHI (B), HhaI (open circles), and HpaII (open triangles) sites in the endogenous H19 gene (open rectangles) and the relevant transgenes are indicated. The BamHI site at −4 kb is unique to the transgenes; thus, probe I identifies a 2-kb transgene-specific BamHI fragment and a 0.6-kb BamHI–HpaII fragment (asterisks) in all transgenes. Probe II does not distinguish between the endogenous and M. spretus H19 transgene. (B and C) Genomic DNA prepared from neonatal livers from N2 transgenic (+) and nontransgenic (−) pups was digested with BamHI (−) alone or together with HpaII (H) or MspI (M). DNAs were hybridized to probe I (B) or probe II (C). The parental origin of the transgene is indicated at the top of the lanes.