Figure 5.

DNA Methylation of the luciferase and H19 transgenes. Genomic DNA was isolated from N2 neonatal liver of transgenic progeny and was digested with BamHI alone (−), BamHI and HpaII (H), and BamHI and MspI (M). The methylation status of HpaII sites at −3 kb was determined using probe I, as described in the legend to Fig. 3. Probe I identifies a transgene-specific 2-kb BamHI fragment (arrowhead) and an 8-kb BamHI fragment from the endogenous locus (closed circle). The transgenic construct and the parental origins of the transgene are indicated at the top of the lanes.