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DNA Methylation of the H19 transgenes in testes. Testes DNA was prepared from a nontransgenic male (Non-tg) and males of the transgenic lines indicated. S, M. spretus; L, −4-kb Luc. The DNA was digested with BamHI (−) or BamHI together with HpaII (H), MspI (M), or HhaI (Hh). The DNA was hybridized to radiolabeled probe I. The sizes of the relevant fragments are indicated on the left. The 2- and 0.6-kb fragments are transgene-specific and common to all transgenes.
