Table 2.
Expression and methylation of H19 and luciferase transgenes
| Transgene | Line no. | Copy no. | Expression† | Methylation pattern‡ |
|---|---|---|---|---|
| M. spretus H19 | S8 | 1 | No | ND |
| S21 | 4 | Mat | Paternal-specific | |
| S25 | 5 | Mat | Paternal-specific | |
| S26 | 2 | No | ND | |
| S28 | 2 | Mat + Pat | Unmethylated | |
| S29 | 2 | Mat + Pat | ND | |
| S30 | 4 | Mat | Paternal-specific | |
| −4-kb Luc | 113 | 21 | Mat + Pat | Unmethylated |
| 138 | 2 | Mat + Pat | Unmethylated | |
| 141 | 2 | Mat + Pat | ND | |
| 142 | 14 | Mat + Pat | Unmethylated | |
| 158 | 1 | No | ND | |
| 167 | 16 | Mat + Pat | Unmethylated | |
| 171 | 17 | Mat + Pat | Unmethylated | |
| −0.8-kb Luc | 82 | 30 | Mat + Pat | ND |
| 94 | 12 | Mat + Pat | ND | |
| 99 | 1 | No | ND | |
| 103 | 8 | Mat + Pat | ND | |
| Δ1H19 | 1–1 | 4 | No | Unmethylated |
| 1–3 | 6 | No | Unmethylated | |
| 1–5 | 3 | No | Unmethylated | |
| 1–18 | 1 | No | Unmethylated |
ND, not determined.
*
The copy number of each transgene was determined as described.
†
Mat indicates transgene expression when inherited maternally and Pat indicates expression when inherited paternally. No indicates that the transgene RNA could not be detected.
‡
Two patterns of DNA methlation of the the 5′ flank of the transgene in neonatal liver were noted: paternal-specific when the transgene was methylated only when paternally inherited, or unmethylated when the pattern was unmethylated irrespective of parental origin.