Figure 6.

Expression patterns of AE20A, 4A7ftz and 3A7ftz lines in Drosophila embryos. β-Gal activity stainings (A–D and G–L) and stainings with an antiserum against EN (C) and CAD (E and F). Lateral view (A) and ventral view (B) of a stage 11 AE20A embryo showing staining in alternate segments (arrowheads). Lateral view (C) and dorsal view (D) of an early stage 11 4A7ftz embryo. (C) The β-gal staining (solid arrowhead) appears more posteriorly of the last en-stripe (open arrowhead). (E and F) Stage 10 (E) and stage 11 (F) embryo stained with antiserum against the cad gene product (arrowheads). (G) Early stage 11 3A7ftz embryo showing ftz-like (open arrowheads) and cad-like (solid arrowhead) expression. (H) Early stage 11 3A7ftz embryo in a presumptive cad¹ mutant background. The cad-like expression (solid arrowhead) is almost absent, whereas the ftz-like expression (open arrowheads) is not affected. (I) 4A7ftz embryo in a presumptive cad³ mutant background showing reduced staining (solid arrowhead). (J) Lateral view and (K) dorsal view. Staining of a late stage 11 4A7ftz embryo after ubiquitous expression of cad. Ectopic expression appears in lateral mesodermal cells (open arrowhead). (L) Early stage 12 4A7ftz embryo after ubiquitous expression of ftz showing ftz-like expression (open arrowheads).