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. 1996 Nov 26;93(24):13890–13895. doi: 10.1073/pnas.93.24.13890

Figure 5.

Figure 5

Chromosomal localization of HRAD9 to 11q13.1–11q13.2. (A) Cy3-labeled genomic HRAD9 was hybridized to methotrexate-synchronized normal human lymphocyte metaphase preparations. The white dual chromatid signals are localized to 11q13 (counterstain 4′,6-diamidino-2-phenylindole/actinomycin D). (Inset) Enlarged version of chromosome 11 from two different metaphase spreads. (B) DNA sequence analysis of the HRAD9 genomic clone used in FISH analysis identifies the location of an intron. The location of the primers used for PCR in sections C and D are indicated. (C) PCR analysis using total human, mouse, or hamster genomic DNA, or DNA isolated from somatic cell hybrids containing a single human chromosome only, as indicated. This confirms the chromosomal location indicated by FISH analysis. (D) PCR analysis sublocalizes HRAD9 to 11q13.1–11q13.2, as the genomic DNA is absent in a somatic cell hybrid containing 11q13.3–11q ter (11936), and present in three hybrids bearing the 11q13.1–11q13.2 region (11943, 13400, and 10482). Arrows to the left of C and D indicate position of relevant DNA fragments.