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. 1996 Nov 26;93(24):13896–13901. doi: 10.1073/pnas.93.24.13896

Figure 1.

Figure 1

The yeast split-hybrid system. (A) Interaction of protein X fused to LexA with protein Y fused to the VP16 transactivation domain (TAD) promotes the expression of TetR which, in turn, binds to tet operators upstream of the HIS3 gene, preventing the growth of yeast in medium lacking histidine. (B) Disruption (depicted by wavy arrow) of the interaction between proteins X and Y prevents expression of TetR, permitting expression of HIS3 and allowing growth of yeast in medium lacking histidine.