Figure 2.

Characterization of the yeast split-hybrid system. The yeast split-hybrid strain YI584 (A and B) are the yeast two-hybrid strain L40 (C) were transformed with the indicated plasmid pairs. Yeast transformants were plated on selective medium plates (lacking tryptophan, uracil, leucine, and lysine) supplemented with histidine and incubated at 30°C for three days. (A) A pool of colonies for each interacting pair of proteins was streaked onto selective medium supplemented with histidine (Left) and onto selective medium lacking histidine (Right). The plates were incubated at 30°C for 3 days. (B) A pool of colonies was seeded in selective media lacking histidine. After incubation of cultures at 30°C for 2 days, growth was measured at OD₆₀₀. Data represent the mean ± SD of two or three experiments and is presented as a percentage of the OD₆₀₀ of yeast grown in selective media supplemented with histidine. (C) A pool of colonies was collected and prepared for β-gal liquid assay. Data represent the mean ± SD of two experiments. A β-gal unit = 1000 × OD₄₂₀/time × protein, where the time is in minutes and the protein is in mg/ml.