Figure 6.

Immunoblots showing the effect of blue light intensities on the blue-light induced decrease of CRY2 protein. (A) Protein samples prepared from wild-type seedlings were collected 1 hr or 24 hr after exposure of the 4-day-old etiolated seedlings to blue light with different fluence rates indicated. (B and C) Samples were prepared from CRY2-overexpressing transgenic seedlings grown in the dark (lane D), under red light with (B lanes) or without (lane R) 1 hr blue light treatment at the fluence rates indicated before harvesting. The immunoblot was probed with anti-CRY2 antibody (B), stripped, and reprobed with anti-CRY1 antibody (C).