Figure 1.
Nondenaturing in-gel hybridization technique to detect single-stranded TG1–3 DNA. In both gels, lanes 1 and 2 contained, respectively, 4 ng and 0.4 ng of single-stranded phagemid DNA derived from pGT75; lanes 3 and 4 contained, respectively, 30 ng and 3 ng of linearized double-stranded pMW75 DNA; and lanes 5 and 6 contained, respectively, 30 ng and 3 ng of linearized pMW75 DNA that was heat-denatured for 10 min at 95°C prior to loading. M denotes end-labeled ladder DNA. The gels were treated for in-gel hybridization. The gel in A was treated as a nondenaturing gel, whereas the gel in B was denatured in the gel prior to hybridization to the end-labeled CA-oligo probe.