Table 4.

Prediction criteria for buried residues

Burial, %	Predicted fraction,* %	Prediction criteria
≥95	0.3	Residue, as well as both flanking residues, are hydrophobic and Hav ≥ 75.
≥90	1.3	Residue is hydrophobic and Hav ≥ 75 or
		Residue, as well as both flanking residues, are hydrophobic and Hav ≥ 65
≥80	6.1	Residue is hydrophobic and any of the following conditions are met:
		(i) Hav ≥ 60 and preceding residue is hydrophobic;
		(ii) Hav ≥ 65 and both flanking residues are hydrophobic;
		(iii) Hav ≥ 70;
		(iv) Hmom ≥ 200 and residues at either (−3 and +4) or (−4 and +3) relative to the residue are hydrophobic

^*Percent of total residues in a protein that are predicted to be buried. These numbers are based on the total number of predicted buried residues found for a given burial cutoff in the original data set of 6143 residues.