Table 8.

Predicted buried residues for staphylococcus nuclease

Residue/substitution predicted	Burial observed in wild-type protein, %	ΔΔG, kcal/mol
Ile-15-Gly	50	−3.3
Leu-36-Gly	99	−5.3
Leu-37-Gly	97	−3.8
Leu-38-Gly	98	−0.6
Val-39-Gly	100	−4.7
Leu-108-Gly	90	−7.2

The experimental value for the change in free energy of unfolding (ΔΔG) when the wild-type residue is substituted by Gly is taken from ref. 18.