Figure 1.

Isolation of human DNAs as circular YACs by using the TAR cloning method. (A) TAR cloning vectors. Presented are pVC39-AAH4, -AAT3, -AAH2, -MAH8, -MAH10, and -LAH2 vectors containing Alu, LINE, or MER sequences. CEN6, centromere; Amp^R, ampicillin-resistance gene. (B) The TAR cloning scheme. Yeast spheroplasts are transformed with human DNA along with a TAR cloning vector. Recombination between repeats in the vector and human DNA leads to the establishment of a circular YAC. The filled-in blocks in human DNA and vectors identify repeated Alu, LINE, or MER sequences.