Figure 2.

Physical characterization of five randomly selected circular human YACs developed by TAR cloning from total human DNA. Chromosomal-size DNA was separated by TAFE in a gel and blot-hybridized with a human DNA probe. Strong signals located at the positions of the starting wells correspond to circular YACs (lanes 2, 4, 6, 8, and 10). A band corresponding to linear molecules was also detected. Irradiation of the plugs with human YACs resulted in appearance of the bands corresponding to linear forms of the YACs (lanes 3, 5, 7, 9, and 11).