Table 1.
TAR vector* | Alu orientation | Mammalian DNA | No. of His+ or Trp+ transformants† |
---|---|---|---|
pVC39-AAT3 | → ← | None | 1 –3 |
-AAH2 | → ← | None | 0 –7 |
-AAH4 | → → | None | 0 –7 |
-AAT3 | → ← | Human | 300 –1000 |
-AAH2 | → ← | Human | 400 –1000 |
-AAH4 | → → | Human | 5 –20 |
-AAH2 | → ← | Hamster | 3 –10 |
-AAH2 | → ← | Mouse | 1 –20 |
-LAH2 | LINE ← | Human | 200 –500 |
-MAH8 | MER ← | Human | 50 –100 |
-MAH10 | MER ← | Human | 100 –200 |
pVC1 | TEL-Alu | Human | 300 –1000 |
pNKBAC39 | → ← | Human | 200 –500 |
Yeast spheroplasts were transformed with 1 μg of linearized plasmids along with 5 μg of human or rodent DNA.
Three to 20 independent transformations were carried out for each condition.