Skip to main content
. Author manuscript; available in PMC: 2007 Aug 13.
Published in final edited form as: J Neurochem. 2001 Sep;78(6):1219–1232. doi: 10.1046/j.1471-4159.2001.00495.x

Fig. 8.

Fig. 8

Lithium restores growth factor stimulation of CREB DNA binding activity in SH-SY5Y cells overexpressing GSK3β. (a) CREB DNA binding activity was measured by EMSA in nuclear extracts prepared from GSK3β-overexpressing cells incubated without serum (24 h) and treated without or with EGF (50 ng/mL; 1 h), or IGF-1 (50 ng/mL; 1 h) alone or following pre-incubation with lithium (Li; 20 mm; 1 h). (b) Quantitative values are expressed as the percent of basal CREB DNA binding activity in serum-starved GSK3β-overexpressing cells. Mean ± SEM, n = 4–6. (c) Nuclear extracts prepared from GSK3β-overexpressing cells incubated without serum (24 h) and treated without or with EGF (50 ng/mL; 1 h), or IGF-1 (50 ng/mL; 1 h) alone or following pre-incubation with lithium (Li; 20 mm; 1 h) were immunoblotted for total levels of CREB.