Table 1.

Sequence of PCR primers used to amplify switch region probes

Probe	Forward primer	Reverse primer	Size, bp	Annealing temperature, °C
5′σμ	CAGATCTGAAAGTGCTCTACTG	CTTATCCTAAAGTGAGTAGTTG	462	50
3′σδ	CACCGAAACCTCTGGAGGGAAG	TGTGCTGGACCACGCATTTG	548	63
5′Sμ	TTTGAAGGAGAGGTCGCACGAG	TCAGCTAAAGCCATCTCATTGCC	631	58
3′Sμ	TCTACACTGCGTTCCCCATCAC	CGTTCTGAGTGCCCTCACTACTTG	593	58
5′Sγ	CAGAATGGTCATAATCGCTGCC	CATCCCGTCATGTTCCTCGTG	719	58
3′Sγ	GCTATTCCAAGACAGGGGGTTCC	CAGAAAGCTTGCAGGACCG	436	55
5′Sα	CAGCATCCAGCCACATCTG	AGATCCTTCCTGCCTGGTTAG	405	58
3′Sα	CATGGTGCAGGAGCTGTGTAAC	TCCACTCTGGTGTGAGTGAAGG	562	58
5′Sɛ	AAGAGAACCTCCCCAGCACTC	GTCGGGTCTCTGACTTCTTGGTCT	558	61
3′Sɛ	CGGGGCTGGATACTGTGATTTTG	TATCATCAGGCTGGGCTCAGGAAG	547	58

The sequence of the primers is given from 5′ to 3′.