Table 2.
IgH switch recombination: Summary of 21 human MM cell lines
| Line | Isotype*
|
14q32 abnormality† | Legitimate switch‡ | Illegitimate switch§¶
|
||
|---|---|---|---|---|---|---|
| H | L | 5′ probe | 3′ probe | |||
| FLAM-76 | — | κ | 11q13‖ | None | None | None |
| MM-M1 | (γ) | (κ) | 11q13‖ | Sμ→σδ, Sμ→Sγ | None | None |
| H1112 | δ | λ | 11‖ | Sμ→σδ, Sμ→Sγ | None | None |
| SK-MM2 | — | κ | 11q13‖ | None | None | Sγ [11q13] |
| KMS-12 | NP | 11q13‖ | Sμ→σδ, Sα→Sγ | Sμ, Sα | Sγ [11q13], Sμ [14]** | |
| Delta-47 | δ | λ | 14q+ | Sμ→σδ, Sμ→Sα, Sγ→Sα | None | None |
| TH | ㇇ | κ | 14q+ | Sμ→Sα | Sμ | None |
| 8226 | (γ) | λ | 1p13 | None | None | Sㆆ |
| MM.1 | (α) | λ | 12q24 | None | Sμ | Sγ |
| JJN3§§ | (α) | κ | 14q+ | None | 5′σμ | Sμ [16q23]§§ |
| SK-MM1 | — | κ | 14q+ | Sμ→Sγ | Sμ | Sμ [6], Sγ |
| JIM3§§ | (α) | λ | 14q+ | None | Sμ | Sγ [4p16]§§ |
| KMS-11 | (γ) | κ | None | None | Sμ | Sμ [4p16], Sμ, Sγ |
| KMM-1 | — | λ | None | None | σμ [8q24] | σδ [21q22] |
| U266 | ɛ | λ | None‖ | Sμ→Sɛ | Sγ | Sα [11q13] |
| H929 | (α) | κ | None | None | Sμ | Sα |
| OPM-2 | (γ) | λ | None | None | Sμ | Sγ |
| ANBL-6 | — | λ | None | None | Sμ | Sμ |
| UTMC-2 | α | κ | None | Sμ→Sα | Sμ | Sμ |
| ark | γ | κ | None | Sμ→Sγ, Sα→Sγ, Sα↔Sμγ | None | None |
| OCI-MY5 | α | λ | ND | Sμ→Sα | None | None |
Isotype of IgH chain present in tumor but absent from cell line indicated by parentheses, from original reference or investigator, as cited. NP, Nonproducer.
Karyotype from original reference as cited, except ark, SK-MM2, and MM-M1; H1112 has an 11;14 translocation by chromosome painting analysis (M.C. and E. Schrock, unpublished data); ND, Not done.
A legitimate switch recombination fragment hybridizes to two switch region probes (see Fig. 1). Productive and downstream switch indicated by →; inversion indicated by ↔.
Cyclin D1 mRNA overexpressed in these lines.
An illegitimate switch recombination fragment hybridizes only to a single 5′ (column 5) or 3′ (column 6) probe and to no other probes.
Chromosomal partner in cloned breakpoint indicated within square brackets.
Unidentifed sequence from chromosome 14 juxtaposed to Sμ.
This cell line expresses alpha mRNA, not gamma mRNA (data not shown).
This illegitimate switch was not detected by any of the 10 probes in Table 1.
By Southern blot analysis the translocation breakpoint identified by this probe was identified in primary tumor corresponding to this cell line.