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. 2003 Aug 18;4:7. doi: 10.1186/1471-2091-4-7

Figure 2.

Figure 2

(A) The Ca2+-affinity of CR56 was determined by rate dialysis [Ca2+]bound/[CR56] versus the free Ca2+ concentration. The concentration of CR56 was 15 μM throughout the analysis. (B) Scatchard analysis of the data shown in panel A. The solid line represents the best fit by least-squares analysis as described in the materials and methods section. The fact that the experimental points are located on a straight line indicates absence of cooperativity between the two Ca2+ binding sites. Strong positive cooperativity would result in upward curvature, while strong negative cooperativity would result in downward curvature of the Scatchard plot. The average affinity is determined to, KD = 10.6 μM assuming two identical sites (n = 2.2).