Figure 4.

Regulation of steroid receptors in strains with Hsp82 derivatives. Strains were cotransformed with expression vectors for the rat GR (rGR) (A), human PR (hPR) (B), and human ER (hER) (C) and the appropriate β-galactosidase reporter gene (pUCΔSS-26X for rGR and hPR; pUCΔSS-ERE for hER). The two panels for rGR represent two separate sets of experiments. The hPR was not tested with Hsp82-(Δ211-259). Hormones were added to low-density cultures from 1000-fold stocks to the indicated final concentrations (logarithmic scale). Inductions were done for 12 h. β-Galactosidase units were corrected for cell density. Data represent the average of the β-galactosidase activities from two yeast clones. DAC, deacylcortivazol.