Figure 5.

Pheromone signaling in strains with Hsp82 derivatives. Strains precultured in YEPD were diluted to a density of 1.2 × 10⁷ cells per ml and streaked onto YEPD plates with and without 5 μM α-factor. The control strain E929-6C-24 (Hsp82 wt, Δste11) is not growth arrested upon exposure to α-factor since it lacks the kinase Ste11, an essential component of the pheromone signaling pathway. The plates were photographed after 3 days of incubation at 30°C.