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. 1996 Nov 26;93(24):13973–13978. doi: 10.1073/pnas.93.24.13973

Figure 2.

Figure 2

TNF-dependent recruitment of TRAF2 and c-IAP1 to TNF-R1 is TNF-R2-independent. (A) Comparison of TNF-R2 expression in U937 and HeLa cells. Lysates of U937 cells (≈1 × 108) or HeLa cells (≈1 × 108) were immunoprecipitated with the 1040 mAb to TNF-R2 (lanes 2 and 4) or mouse IgG control (lanes 1 and 3). The immunoprecipitates were subsequently analyzed by immunoblotting with a polyclonal TNF-R2 antibody. Positions of molecular mass standards (in kDa) are shown on the left. (B and C) Recruitment of TRAF2 and c-IAP1 to the TNF-R1 complex in U937 and HeLa cells. TNF (100 ng/ml) was used to treat 2 × 108 U937 cells (lanes 1 and 2) or HeLa cells (lanes 3 and 4) for 5 min (lanes 2 and 4). Lanes 1 and 3 were left untreated. Cell lysates were immunoprecipitated with the 985 mAb to TNF-R1 and immunoprecipitates were analyzed by immunoblotting with polyclonal antibodies against TRAF2 (B) or c-IAP1 (C). Positions of molecular mass standards (in kDa) are shown on the left.