Figure 3.

TRADD and TRAF2 mediate recruitment of c-IAP1 to TNF-R1. Combinations of expression vectors for TNF-R1, myc tagged c-IAP1, TRADD, and TRAF2 (as indicated) were used to transfect 293 cells (≈2 × 10⁶). Transfected cell lysates were immunoprecipitated with either mouse IgG control (lanes 1, 3, 5, and 7) or a goat anti human TNF-R1 antibody (lanes 2, 4, 6, and 8). Immunoprecipitates were analyzed for c-IAP1 by immunoblotting with a myc epitope antibody. Positions of molecular mass standards (in kDa) are shown on the left.