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Biochemical properties of TRADD, TRAF2, and c-IAP1 in HeLa cells. HeLa cell lysates (1 × 108 cells) were separated by size exclusion chromatography on Superdex 200 column. Individual fractions were analyzed by immunoblotting with polyclonal antibodies against TRADD, TRAF2, or c-IAP1 as indicated. Positions of molecular mass standards (in kDa) are shown on the left.
