Figure 5.
Association of TRAF2 and c-IAP1 in HeLa cells. HeLa cells (≈2 × 108) were treated with TNF (100 ng/ml) for 5 min (lanes 2, 4, 6, and 8) or left untreated (lanes 1, 3, 5, and 7). Cell lysates were immunoprecipitated with rabbit polyclonal antisera against TRADD (lanes 1 and 2), TRAF1 (lanes 3 and 4), TRAF2 (lanes 5 and 6), or c-IAP1 (lanes 7 and 8). Immunoprecipitates were analyzed by immunoblotting with a polyclonal antibody against c-IAP1. Positions of molecular mass standards (in kDa) are shown on the left.