Table 1.

Primer	Sequence a(position, specificity, and polarity)
First PCR
P1	5'-TCA CCA TAT TCT TGG GAA CAA GA-3' (nt 2823–2845, universal, sense)
S1-2	5'-CGA ACC ACT GAA CAA ATG GC-3' (nt 685–704, universal, antisense)
Second PCR
Mix A
B2	5'-GGC TCM AGT TCM GGA ACA GT-3' (nt 67–86, types A to E specific, sense)
BA1R	5'-CTC GCG GAG ATT GAC GAG ATG T-3' (nt 113–134, type A specific, antisense)
BB1R	5'-CAG GTT GGT GAG TGA CTG GAG A-3' (nt 324–345, type B specific, antisense)
BC1R	5'-GGT CCT AGG AAT CCT GAT GTT G-3' (nt 165–186, type C specific, antisense)
Mix B
BD1	5'-GCC AAC AAG GTA GGA GCT-3' (nt 2979–2996, type D specific, sense)
BE1	5'-CAC CAG AAA TCC AGA TTG GGA CCA-3' (nt 2955–2978, type E specific, sense)
BF1	5'-GYT ACG GTC CAG GGT TAC CA-3' (nt 3032–3051, type F specific, sense)
B2R	5'-GGA GGC GGA TYT GCT GGC AA-3' (nt 3078–3097, types D to F specific, antisense)

^a An "M" represents a nucleotide that could be either an A or a C; a "Y" represents a nucleotide that could be either a C or a T. nt: nucleotide.