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. 2007 Jul 11;4:73. doi: 10.1186/1743-422X-4-73

Figure 1.

Figure 1

Tet-dependent replication of vvtetOA12L and one-step growth curve. a. Tet-dependent replication of vvtetOA12L. T-REx 293 cells were infected with vvtetOA12L at an MOI of 1 PFU/cell in the presence of tetracycline (Tet) at various concentrations of 0, 10, 20, 30, and 40 μg/mL. The infected cell extracts harvested at 24 hpi were titered on BSC 40 cells to determine the virus yields. b. One-step growth curve. T-REx 293 cells were infected with vvtetOA12L in the presence and absence of Tet (30 μg/mL) and harvested at 3, 5, 8, 12, and 24 hpi. Each virus titer (PFU/ml) was scaled in log phase.