Table 2.
Identified phosphoproteins of M. genitalium
| Spot | Protein name | Gene ID; Protein ID & Locus tag | Theoretical pI/Mr | Peptide mass fingerprinting | ||
| Match peptides a) | Sequence coverage coverage (%) | MS & MS/MS score b) | ||||
| G1 | Putative structural protein involved in cytoskeleton | 875488; C64236 (MG328) | 4.61/88353.9 | 26 (1) | 39 | 811 |
| G2 | Heat shock protein dnaJ homolog MG200 | 875694; A64222 (MG200) | 4.86/68494.8 | 17 (2) | 30 | 396 |
| G3 | P65 protein | 875361; I64223 (MG217) | 4.59/44636.8 | 17 (1) | 33 | 464 |
| G4 c) | Conserved hypothetical protein | 875359; AAC71439 (MG218.1) | 4.96/39701.4 | 17(3) | 51 | 461 |
| G5 | DNA-directed RNA polymerase delta chain | 875707; D64202 (MG022) | 4.48/17046.3 | 6 (0) | 55 | 204 |
| G6 | Transcription elongation factor greA homolog | 875338; B64231 (MG282) | 4.99/18151.5 | 13 (1) | 81 | 857 |
| G7 | Heat shock protein GroEL | 875662; D64243 (MG392) | 5.82/58318.4 | 37 (2) | 67 | 1030 |
| G8 | DnaK-type molecular chaperone | 875435; G64233; (MG305) | 6.79/65010.5 | 28 (0) | 46 | 997 |
| G9 | 29 (1) | 44 | 1120 | |||
| G10 | 26 (2) | 45 | 1160 | |||
| G11 | Cytadherence-accessory protein | 875571; A64235 | 7.01/ | 22 (2) | 40 | 401 |
| G12 | (hmw3) homolog | (MG317) | 68679.1 | 24 (2) | 42 | 515 |
| G13 | Pyruvate dehydrogenase E1-alpha chain | 875404; C64230 (MG274) | 5.55/40625.3 | 13 (1) | 45 | 651 |
| G14 | Phosphopyruvate hydratase -(enolase) | 875644; A64245 (MG407) | 6.63/49987.9 | 15 (2) | 45 | 297 |
| G15 | Translation elongation factor EF-Tu | 875605; EFYMTG (MG451) | 6.18/42963.3 | 14 (2) | 44 | 582 |
| G16 | Restriction-modification enzyme | 875615; D64248 | 7.58/ | 26 (6) | 52 | 770 |
| G17 | EcoD specificity chain | (MG438) | 44082.8 | 23 (6) | 46 | 409 |
| G18 | Pyruvate dehydrogenase E1-beta | 875399; B64230 | 6.04/ | 13 (1) | 41 | 697 |
| G19 | chain | (MG273) | 36002.8 | 13 (1) | 41 | 749 |
| G20 | Phosphate acetyltransferase | 875564; A64233 (MG299) | 6.97/35446.5 | 13 (0) | 47 | 575 |
| G21 c) | Hypothetical protein | 875676; G64241 (MG377 | 6.24/22635 | 27(5) | 74 | 864 |
| G22 c) | 20(3) | 66 | 642 | |||
| G23 | Methionine sulfoxide reductase A | 875645; B64245 (MG408) | 6.18/18403.2 | 6 (1) | 33 | 147 |
| G24 | Conserved hypothetical protein | 875626; B64247 (MG427) | 6.19/ | 7(1) | 82 | 220 |
| G25 | 15593 | 4(0) | 30 | |||
| G26 | 7(1) | 82 | ||||
| G27 | Hypothetical protein MG342 | 875477; H64237 (MG342) | 8.37/ | 8 (1) | 56 | 248 |
| G28 | 19115.9 | 4 (1) | 30 | 82 | ||
| G29 c) | ATP synthase alpha chain | 875650; (MG401) | 6.70/57215.4 | 22(6) | 55 | 220 |
| G30 c) | L-lactate dehydrogenase and | 875596; (MG460) | 8.76/33966.2 | 28(1) | 72 | 566 d) |
| Ribosomal protein S2 | 875215 (MG070) | 9.36/32746.8 | 17(4) | 46 | ||
| G31 c) | L-lactate dehydrogenase | 875596; (MG460) | 8.76/33966.2 | 25(3) | 70 | 396 |
a) Number of peptides that match the theoretical digest of the primary protein identified. The number in parentheses is the count of potentially phosphorylated peptides found by Mascot and FindMod (see additional file 1: Putative_Phosphopeptides.pdf, Table A).
b) Combined score for the peptide mass fingerprint including both MS and MS/MS peptide matches for the spectrum. Scores of 95 or greater were considered significant (p < 0.05).
c) Identified proteins detected by labeling with 33P-phosphoric acid.
d) Spot G30 was identified to contain both L-lactate dehydrogenase and Ribosomal protein S2. The MS & MS/MS score shown here belongs to the L-lactate dehydrogenase.