Figure 2.

Autoradiograms of the Southern blot analysis of the presence of Rxfp1 and Rxfp2 mRNA in the vas deferens (NW = not washed, W = washed to remove spermatozoa) and in spermatozoa removed from the cauda epididymis. The amount of cDNA used from the vas deferens was 2 μL. Exposure to film was 2 h for Rxfp1 and 1 h for Rxfp2 transcripts. Results are representative of 3 independent determinations, each one with a different animal.