Figure 1.

Variations of the Lκ transgene construct. (a) The portion of the VκOx1 where WT and ΔBWT and mutants differ has been expanded. WT and ΔBWT are identical to Lκ and LκΔB (19, 23). Modified bases shown in boldface lowercase type. Potential hairpins and palindromic sequences are underlined. (b) Genetic map of LκWTM7 mouse. One of the V segments is WT and the other M7. Both hypermutate implying that the 3′ enhancer drives the process when placed upstream of the VJ segment. The restriction endonuclease cleavage sites are abbreviated as follows: R, EcoRI; B, BamHI; N, NotI.