Table 1.

Transgene mutations in PCR clones derived from Peyer’s patches germinal center B cells

Mouse line and transgene	No. of clones*		Total PM†	PMs per 103 base pairs‡
	Total	>1 PM		Total	>1 PM
LκWTM7
WT	88	34	184	7.4	17.3
M7	81	44	211	9.2	16.0
LκNG
ΔBWT + ΔBWTm	59	33	197	11.8	20.1
ΔBM7	26	12	52	7.1	13.6
PCR error	245	1	29	0.4
LκΔBTCAM7/ΔLi
ΔBTCAM7	172	48	267	5.5	18.5
PCR error	52	0	2	0.1

PM, point mutation. 

^*Clones sequenced, and number of those with more than one point mutation (>1 PM). 

^†Sum of point mutations in all clones. 

^‡Frequency of PMs per 10³ base pairs computed for all clones or for those with two or more mutations.