Table 2.
Transgene mutations in PCR clones derived from anti-phOx hybridomas
| Hybridoma | Antibody
|
No. of PMs per
copy*
|
||||||
|---|---|---|---|---|---|---|---|---|
| Cκ, rat | Cκ, mouse | ΔBWT | ΔBWTm† | ΔBM7 | WT | M7 | ΔBTCAM7 | |
| LκNG | ||||||||
| C15.2.7 | + | − | 8‡ and 6 | 7 | 3 and 3 | |||
| C10.5.4 | + | − | 5‡ and 8 | 6 | 1 and 1 | |||
| A6.8 | − | + | 3 and 3 | 1 | 0 | |||
| C23.1.5 | + | − | 7‡ and nf | NF | 2 and 2 | |||
| D1.1.5 | + | − | 10‡ and 0 | NF | 10 and 0 | |||
| B20.6 | + | − | 4 and 0 | 2 | 4 and 0 | |||
| C6.3.6 | + | − | 8‡ and nf | 4 | 5 and 2 | |||
| D12.3.8 | + | − | 0 | 2 | 0 | |||
| C4.6.8 | + | − | 0 | 2 | 0 | |||
| D23.4 | + | − | 1 and 0 | 1 | 1 and 0 | |||
| LκWTM7 | ||||||||
| D3.3.3 | + | − | 3§ | 7‡ | ||||
| C12.1.3 | + | − | 5§ | 7‡ | ||||
| EH2.5 | ± | + | 0 | 4 | ||||
| RB3.6 | ± | + | 0 | 3 | ||||
| EA8.7 | ± | + | 0 | 1 | ||||
| C3.23.2 | ± | + | 0 | 0 | ||||
| C11.4.8.8 | + | − | 0 | 0 | ||||
| LκΔBTCAM7/ΔLi | ||||||||
| B2.4.4 | + | − | 3 | |||||
| C14.1.9 | + | − | 6‡ | |||||
| A20.5.2 | + | − | 4‡ | |||||
PM, point mutation; NF, not found.
*
Number of PMs found in each transgenic copy.
†
This copy was identified by a silent substitution (G for A) in codon Lys-18.
‡
Copies containing the critical mutation of His-34 to Asn or Gln. In addition, all of them included the mutation Tyr-36 to Phe.
§
These copies also carried deletions that result in a coding sequence out of frame.