Table 1.
Target | Primer Sets | |
forward | reverse | |
α-tubulin1) | cacccgtcttcagggcttcttggttt | catttcaccatctggttggctggctc |
GAPDH2) | gaaggtgaaggtcggagtc | gaagatggtgatgggatttc |
β-actin3) | atggatgatgatatcgccgcg | ctagaagcatttgcggtggacg |
7SL3) | gggctgtagtgcgctatgc | cccgggaggtcaccatatt |
Vif | gatggcaggtgatgattgtgtgg | ctgtccattcattgtatggc |
hY14) | ggctggtccgaaggtagtga | aaagactagtcaagtgcagtagtgag |
hY34) | ggctggtccgagtgcagtg | aaaggctagtcaagtgaagcagtgg |
hY44) | ggctggtccgagtgcagtg | aaagccagtcaaatttagcagtggg |
hY54) | agttggtccgagtgttgtggg | aaaacatgcaagctagtcaagcgcg |
U15) | cctggcaggggagataccatgatcacg | ggggaaagcgcgaacgcagtccccc |
U25) | cttcttggccttttagctaagatc | ggtgcactgttcctggaggtactgc |
U45) | gctttgcgcagtggcagtatcg | cagtctccgtagagactgtcaaaaattg |
U65) | gtgctcgcttcggcagcacatatac | ggaacgcttcacgaatttgcg |
1) Eppendorf cMaster RTplusPCR system (Eppendorf Inc. Westbury, NY)
2) Funaki et al. 2003 [54]
3) Onafuwa-Nuga et al. 2006 [28]
4) Chiu et al 2006 [22]
5) Giles et al. 2004 [41]