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. Author manuscript; available in PMC: 2008 Jul 1.
Published in final edited form as: J Virol Methods. 2007 Apr 9;143(1):104–111. doi: 10.1016/j.jviromet.2007.02.015

Figure 3.

Figure 3

Infectivity of pseudovirions derived from pPCR from patient 05ZM373. (A) PCR and pPCR amplification of env genes from patient 05ZM373 plasma. Five HIV-1 rev/env cassettes were obtained using the single genome amplification (SGA) method. The CMV promoter was amplified and then added to all five env genes at the 5′ end by pPCR. (B) The pPCR DNA was cotransfected with pSG3Δenv into 293T cells. Pseudovirions were harvested 48 hrs after transfection and were used to infect TZM-bl cells. The luciferase activity was measured two days after infection. The data is shown as the mean ± standard error (n = 4 independent assays).