Table 3.
Comparison of TCID50 titers of pseudovirions harvested from different cell cultures.
pPCR DNA | Luciferase Activity (RLU) | TCID50/ml | Volume | Total TCID50 | |
---|---|---|---|---|---|
24 well | 200 ng | 296 610 | 13 975 | 0.5 ml | 6 988 |
6 well | 200 ng | 262 924 | 10 687 | 2 ml | 21 374 |
T25 | 1 000 ng | 460 090 | 69 877 | 5 ml | 349 385 |
T75 | 4 000 ng | 423 603 | 91 376 | 24 ml | 2 193 024 |