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. Author manuscript; available in PMC: 2008 Jul 1.
Published in final edited form as: J Virol Methods. 2007 Apr 9;143(1):104–111. doi: 10.1016/j.jviromet.2007.02.015

Table 4.

Comparison of neutralization titers of pseudovirions generated from HIV-1 env plasmids and pPCR products

Pseudovirus ID50 in TZM-bl cells
Reciprocal serum dilution
μg/ml
BB8 BB12 BB28 BB55 BB70 BB106 Normal Sera 2G12 2F5 4E10
CAP210.2.00.E8 plasmid <20 24 52 46 292 <20 <20 >50 >50 <2
pPCR <20 <20 25 <20 147 <20 <20 >50 >50 <2
DU422.1 plasmid 35 320 93 67 279 74 <20 >50 >50 <2
pPCR 30 249 79 37 197 44 <20 >50 >50 <2
ZM53.MPB12 plasmid <20 <20 390 <20 <20 22 <20 >50 >50 6
pPCR <20 <20 279 <20 <20 <20 <20 >50 >50 11
ZM109.FPB4 plasmid 103 49 44 46 282 <20 <20 >50 >50 3
pPCR 69 38 31 40 168 <20 <20 >50 >50 2
TRO.11 plasmid >540 >540 40 208 104 90 <20 <2 >50 <2
pPCR 293 402 21 152 41 77 <20 <2 >50 <2