Table 5.

Comparison of different methods used for functional analysis of HIV-1 env quasispecies populations from one infected individual

	Bulk PCR	SGAa	pPCR
PCR reaction	one	multiple	multiple
Ligation	single	multiple	no
Transformation	single	multiple	no
Plasmid DNA preparation	multiple	multiple	no
Recombination	yes	no	no
Resampling	yes	no	no
Time needed for transfection	days	weeks	hours

^asingle genome amplification